Journal: European Journal of Immunology
Article Title: Spatially Resolved Profiling of Compartmentalized Muscle and Brain Inflammation
doi: 10.1002/eji.70119
Figure Lengend Snippet: Conceptual framework for spatially informed tissue profiling. High‐throughput molecular phenotyping via single‐cell or single‐nucleus RNA sequencing enables the characterization of expression in each cell. Cell types or states can be clustered following dimensional reduction, most commonly UMAP. Individual genes or small subsets can be localized in spatial transcriptomic datasets. At the same time, transcriptional identities (like cell types) can be projected onto spatial maps, integrating both modalities. From there, several downstream applications may be used. Including Single gene validations using orthogonal imaging‐based methods like FISH or IHC Functionally validate spatial findings in culture models in vitro or in animal models in vivo Expand the analysis to incorporate other modalities (omics) that in turn may be integrated. Created with Biorender.com.
Article Snippet: High‐resolution spatial transcriptomic atlas of mouse soleus muscle: Unveiling single cell and subcellular heterogeneity in health and denervation , Jer‐En Hsu , 2024 (preprint) , Spatial Transcriptomics ( Seq‐Scope 3–7 dpi) paired with Histology , Mouse (6 months) , Denervation (surgical nerve removal) , Soleus (with and without sciatic nerve) , Novel hybrid fibers at a transitional state Mapped NMJ nuclei Fiber differences to stress and Denervation‐induced ECM remodeling dynamics.
Techniques: High Throughput Screening Assay, RNA Sequencing, Expressing, Imaging, In Vitro, In Vivo